16117

Kit Contents: 

GST-Raf1-RBD, 2.4mg, contains 2-3mg/mL in 50mM Tris•HCl, pH 7.2, 150mM NaCl, 0.5% Triton® X-100, 5mM MgCl2, 1mM DTT and 10% glycerol; GST-Raf1-RBD interacts with Ras from human and mouse, and possibly from all mammalian species, store at -70°C 

100X GTPyS, 50µL, 10mM in sterile water, store at -70°C (or -20°C) 

100X GDP, 50µL, 100mM in sterile water, store at -70°C (or -20°C) 

Anti-Ras Antibody, 250µL (5 units) mouse monoclonal Ig2ak; Anti-Ras antibody reacts with Ras of human, rat, and mouse; store at 4°C. Note: One unit of Anti-Ras antibody is defined as the amount of antibody required to efficiently detect Ras in 20µg NIH3T3 whole cell lysate by Western blotting (8.5 x 7.5cm membrane). 

Glutathione Resin, 3.0mL, supplied as 50% slurry containing 0.05% sodium azide, store at 4°C 

1X Lysis/Binding/Wash Buffer, 100mL, contains 25mM Tris•HCl, pH 7.2, 150mM NaCl, 5mM MgCl2, 1% NP-40 and 5% glycerol, store at 4°C 

2X SDS Sample Buffer, 1.5mL, contains 125mM Tris•HCl, pH 6.8, 2% glycerol, 4% SDS (w/v) and 0.05% bromophenol blue, store at 4°C 

Spin Cups, 30 each, maximum volume 850µL, store at room temperature or 4°C 

Collection Tubes, 90 each, store at room temperature or 4°C